miltenyi auto macs (Miltenyi Biotec)
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Miltenyi Biotec
miltenyi auto macs
Miltenyi Auto Macs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/auto+macs+pro/autoMACS+Pro+Separator+-+Starter+Kit/pm41544679-116-4-7
Average 96 stars, based on 109 article reviews
Miltenyi Auto Macs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/auto+macs+pro/autoMACS+Pro+Separator+-+Starter+Kit/pm41544679-116-4-7
Average 96 stars, based on 109 article reviews
miltenyi auto macs - by Bioz Stars,
2026-09
96/100 stars
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Blocking Assay:Article Title: Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Article Snippet: The plug was dispersed by refluxing through the needle and the suspension centrifuged 680 x g for 5 minutes at 4 C. Cells were then lysed with lysis buffer (0.17M NH4Cl, 1mM EDTA, 10mM NaHCO3) at room temperature for 5 minutes, washed with 2 volumes PBS+2%FCS, and centrifuged at 680 x g for 5 minutes at 4 C. Cells were resuspended in PBS + 2%FCS and filtered through 40 mm nylon mesh (BD Biosciences). .. Cells were again centrifuged 680 x g for 5 minutes at 4 C and treated with anti-CD16/32 antibody for Fc-receptor block (2 mL/mouse) for 5 minutes at 4 C followed by addition of anti-c-Kit magnetic beads (Miltenyi) at a 1/5 volume/volume ratio for 15 minutes at 4 C. After removing the antibody with two PBS+2%FCS washes, c-Kit-positive cells were isolated using Magnetic Beads:Article Title: Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Article Snippet: The plug was dispersed by refluxing through the needle and the suspension centrifuged 680 x g for 5 minutes at 4 C. Cells were then lysed with lysis buffer (0.17M NH4Cl, 1mM EDTA, 10mM NaHCO3) at room temperature for 5 minutes, washed with 2 volumes PBS+2%FCS, and centrifuged at 680 x g for 5 minutes at 4 C. Cells were resuspended in PBS + 2%FCS and filtered through 40 mm nylon mesh (BD Biosciences). .. Cells were again centrifuged 680 x g for 5 minutes at 4 C and treated with anti-CD16/32 antibody for Fc-receptor block (2 mL/mouse) for 5 minutes at 4 C followed by addition of anti-c-Kit magnetic beads (Miltenyi) at a 1/5 volume/volume ratio for 15 minutes at 4 C. After removing the antibody with two PBS+2%FCS washes, c-Kit-positive cells were isolated using Article Title: Mitochondrial transfer to host cells from ex vivo expanded donor hematopoietic stem cells Article Snippet: .. C-kit positive cells were enriched using anti-APC magnetic beads with Article Title: Myelodysplastic syndromes disable human CD271 + VCAM1 + CD146 + niches supporting normal hematopoietic stem/progenitor cells Article Snippet: .. Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads with Isolation:Article Title: Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Article Snippet: The plug was dispersed by refluxing through the needle and the suspension centrifuged 680 x g for 5 minutes at 4 C. Cells were then lysed with lysis buffer (0.17M NH4Cl, 1mM EDTA, 10mM NaHCO3) at room temperature for 5 minutes, washed with 2 volumes PBS+2%FCS, and centrifuged at 680 x g for 5 minutes at 4 C. Cells were resuspended in PBS + 2%FCS and filtered through 40 mm nylon mesh (BD Biosciences). .. Cells were again centrifuged 680 x g for 5 minutes at 4 C and treated with anti-CD16/32 antibody for Fc-receptor block (2 mL/mouse) for 5 minutes at 4 C followed by addition of anti-c-Kit magnetic beads (Miltenyi) at a 1/5 volume/volume ratio for 15 minutes at 4 C. After removing the antibody with two PBS+2%FCS washes, c-Kit-positive cells were isolated using Article Title: Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis Article Snippet: Anti-c-Kit magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, Cat# 130-091-224) were added at a 1:5 v/v ratio for 15 min at 4 °C. .. After removing the antibody with two PBS +2% FCS (or PBS +0.1% BSA for tracer experiments) washes, c-Kit-positive cells were isolated using Article Title: Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis Article Snippet: .. c-Kit-positive cells were isolated using Article Title: Disease biomarkers and treatment methods related thereto Article Snippet: .. Samples were thawed and CD34+ hematopoietic cells were isolated 20-24 hours later using an Indirect CD34 MicroBead kit (Miltenyi) and an Magnetic Cell Separation:Article Title: Mitochondrial transfer to host cells from ex vivo expanded donor hematopoietic stem cells Article Snippet: .. C-kit positive cells were enriched using anti-APC magnetic beads with Article Title: Myelodysplastic syndromes disable human CD271 + VCAM1 + CD146 + niches supporting normal hematopoietic stem/progenitor cells Article Snippet: .. Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads with Article Title: Disease biomarkers and treatment methods related thereto Article Snippet: .. Samples were thawed and CD34+ hematopoietic cells were isolated 20-24 hours later using an Indirect CD34 MicroBead kit (Miltenyi) and an Labeling:Article Title: Myelodysplastic syndromes disable human CD271 + VCAM1 + CD146 + niches supporting normal hematopoietic stem/progenitor cells Article Snippet: .. Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads with |